Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities

CASTILLO DE LAS MERCEDES JULIETA; GOTTLIEB, ALEXANDRA MARINA; TABOGA, OSCAR ALBERTO; COSTA, HERNÁN; CAMINATA LANDRIEL, SOLEDAD; FERRAROTTI, SUSANA ALICIA

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS.

ACAD BRASILEIRA DE CIENCIAS

Lugar: Rio de Janeiro; Año: 2018 vol. 91

0001-3765

Cyclodextrin glycosyltransferases(CGTases) are important enzymes in the biotechnology field because theycatalyze starch conversion into cyclodextrins and linear oligosaccharides, whichare used in food, pharmaceutical and cosmetic industries. The CGTases areclassified according to their product specificity in α-, β-, α/β- andγ-CGTases. As molecular markers are the preferred tool for bacterialidentification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterialstrain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogeneticrelationship of CGTases along bacterial evolution. The results obtained hereallowed us to identify the strain DF 9R as Paenibacillusbarengoltzii, and to unveil a complex origin for CGTase types during archaealand bacterial evolution. We postulate that the α-CGTase activity represents theancestral type, and that the γ-activity may have derived from β-CGTases.