IFIS - LITORAL   24734
INSTITUTO DE FISICA DEL LITORAL
Unidad Ejecutora - UE
artículos
Título:
Spin label EPR suggests the presence of cholesterol rich domains in cultured insect cell membranes
Autor/es:
GIORIA, V.V.; CLAUS, J.D.; BAQUÉ, M.A.; CASADO, N.M.C.; MICHELOUD, G.A.; GENNARO, A.M.
Revista:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2018 vol. 505 p. 1038 - 1042
ISSN:
0006-291X
Resumen:
Different spin labels were incorporated to the membranes of cultured insect UFL-AG-286 cells in order tocharacterize their physical properties by Electron Paramagnetic Resonance spectroscopy (EPR). Thespectrum of the spin label 12-SASL incorporated to cell membranes was similar as those obtained inmembrane model systems composed of eggPC/cholesterol. However, the spectrum of the spin label CSL,chemically related to cholesterol, was drastically different in the two systems. Interestingly, when cellcholesterol content was reduced using methyl beta cyclodextrin, an EPR spectrum similar to those ofmodel membranes was obtained. The analysis of these experiments suggests the existence of cholesterolrich regions in UFL-AG-286 cell membranes.