Evaluation of growth hormone receptor and intracellular signal transduction pathway proteins in a follicular persistence model in cattle

LEIVA, CRISTIAN J.M.; BELOTTI, E, MATIAS; ORTEGA, HUGO H.; MATILLER, VALENTINA; SALVETTI, NATALIA; DURANTE, LEANDRO; DIAZ, PABLO U.; MARELLI, BELKIS E.

Mar de Plata

Congreso; LXIV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2019

Sociedad Argentina de Investigación Clínica (SAIC)

Esperanza, Santa Fe, ArgentinaFollicular persistence is a component of cystic ovarian disease (COD) in dairy cows. Numerous events occur during folliculogenesis and an adequate supply of nutrients is necessary. Although growth hormone (GH) has been associated with follicular development, little is known about its function in ovarian diseases. The aim of this study was to evaluate the immunoexpression of GH receptor (GHR), the intracellular signal transduction pathway proteins Janus kinases (JAKs) and the signal transducers and activators of transcription (STATs) by immunohistochemistry, during follicular development in the ovary of cows with induced persistence. The experimental model was performed with an intravaginal progesterone device to get subluteal plasma concentrations of the hormone, obtaining anovulatory persistent follicles. Cows were ovariectomized at the expected day of ovulation (P0) and at 5 (P5), 10 (P10) and 15 days (P15) of persistence (n=5). Control cows were ovariectomized in proestrous to obtain preovulatory dominant follicles (C; n=5). In granulosa cells, the immunoexpression of GHR decreased during folliculogenesis as follicular growth progressed both in control and persistence groups. In theca interna, the expression was lower in antral follicles of C group than in antral follicles of all persistence groups (p