Evaluation of Immunological Parameters in Caiman latirostris Embryos Exposed to Different Pesticides Mixtures

LÓPEZ GONZÁLEZ EVELYN C.; AMAVET PATRICIA SUSANA; MESTRE, ANA P.; POLETTA, GISELA L.; MESTRE, ANA P.; POLETTA, GISELA L.; CARLETTI, JULIETA; CARLETTI, JULIETA; ODETTI, LUCÍA; ODETTI, LUCÍA; SIROSKI, PABLO ARIEL; SIROSKI, PABLO ARIEL; LÓPEZ GONZÁLEZ EVELYN C.; AMAVET PATRICIA SUSANA

Santa Fe

Congreso; 25th Working Meeting of the Crocodile Specialist Group (SSC-IUCN); 2018

Crocodile Specialist Group (SSC-IUCN)/Universidad Nacional del Litoral

The aim of this study was to evaluate the potential effects of embryonic exposure to different pesticide mixtures on immune system of C. latirostris. The study was performed in the ?Proyecto Yacaré? (Gob. Santa Fe/MUPCN) facilities. Sixty eggs from 5 different nests were equitatively distributed into 4 experimental groups and exposure to pesticide mixtures by spraying the incubation material in the early stages of embryonic development: 1) vehicle control (3.5 ml of ethanol); 2) Glyphosate (Roundup Full II® -GLY- 2%) + Chlorpyrifos (Lorsban* 48E® -CPF- 0.8%); 3) Chlorpyrifos (Lorsban* 48E® -CPF- 0.8%) + Cypermethrin (Atanor® -CYP- 0.15%); 4) Glyphosate (Roundup Full II® -GLY- 2%) + Cypermethrin (Atanor® -CYP- 0.15%). The eggs were incubated in an artificial incubator, under controlled conditions of temperature of 31 ± 1 °C and 95% humidity. At the hatching time, blood samples were obtained from the spinal vein of all neonates; and aliquots of the collected blood were used for measures of total (TWBC) and differential (DWBC) white blood cell count. The remaining sample was centrifuged at 2500 × g for 15 min and stored at -80°C until be used for the determination of Natural Antibodies levels (NAb) titers. The provisional results for the analyzed parameters (TWBC, DWBC and NAb titers) no showed differences between exposed and control animals. This could be consequence of a lower sensibility of these variables in C. latirostris. However, it could also be expected that successive exposures can generate a significant difference. These results suggest that a higher number of parameters must be incorporate for a better understanding of the immune response of C.latirostris when they are exposed to the aforementioned agrochemicals.