Altered expression of matrix metalloproteinase 14 in the ovary of cows with acth-induced stress and with cystic ovarian disease (COD).

NOTARO, U.S.; ORTEGA HH.; MATILLER V.; SALVETTI NR.; VARELA M.L.; BELOTTI, E.M.

Mar del Plata

Congreso; LXI Reunion Científica Anual de la Sociedad Argentina de Investigacion Clinica (SAIC).; 2018

SAIC

Dairy cattle are daily subjected to stressful situations some of which have been associated to reproductive disorders. Under these conditions, the microenvironment of the ovary is modified, affecting directly their function. One of the important functions influenced by external stressors is the extracellular remodeling, necessary to ovulation. The aim of this study was to evaluate the expression of matrix metalloproteinase (MMP) 14, a protein involved in tissue remodeling during ovulation. MMP14 expression was analyzed by immunohistochemistry on ovarian samples of cows treated with 100 IU of adreconcorticotropin (ACTH) every 12 hours, for four days before ovulation time, and cows with cystic ovarian disease (COD). Granulosa and theca interna cells of primary, small preantral, large preantral, antral, atretic and preovulatory follicles of treated animals (n=7), control group (n=5) and from cows with spontaneous COD (n=7) were evaluated. We detected that granulosa cells of all follicular structures from spontaneous COD, presented higher expression than those of ACTH-treated and control groups in all categories (p