Color doppler, ultrasound and pathologic features of spontaneous Leydig Cell Carcinoma of testis in Balb/c CmedC mice.

SALVETTI NR; BELOTTI EM; ORTEGA HH; BERTONA JJ; SALINAS JF; SACCO SC

Washington, DC

Congreso; 2018 ACVP and ASVCP Concurrent Annual Meeting; 2018

ACVP and ASVCP

Background: Carcinomas of the testis occur spontaneously in Balb/c mice. The color doppler sonographic appearance of Leydig cell carcinoma (LCC) has not been described in mice.Objective: To describe the ultrasound, color Doppler and pathologic features of spontaneous LCC of the testis in Balb/c CMedC mice. Methods: Two, 11 and 12-month-old, respectively, Balb/c CMedC male mice with a history of aggression, anorexia, depression and ascites were submitted for diagnostic work up. The testicles were enlarged, reddish and bluish; they were examined with a color Doppler ultrasoundZ6Vet. Necropsies were performed and selected tissues were collected for histopathology.Results: Ultrasound showed a large, multinodular, oval mass of heterogeneous echotexture in one testicle of each mouse. Color doppler revealed central and peripheral, intense hyper-vascularization. Grossly, the tumors were unilateral white to yellowish nodules (10-13 mm diameter) with multifocal cystic cavities and hemorrhagic areas. Histology showed a unencapsulated, multilobulated and densely-cellular neoplasm. This mass was composed of sheets of polyhedral cells with eosinophilic, granular or vacuolated cytoplasm. The cells were supported by a fine fibrovascular stroma. A central round nucleus with stippled chromatin and 1-3 magenta nucleoli was present. Marked anisocytosis and anisokaryosis was also observed. Mitotic rate was ~ 1 per 10 HPF. Metastases were observed in the testicular vein and epididymis of one mouse and in the liver of the other. Conclusion: This report confirms that testicular ultrasound is very useful for the diagnosis of tumors and color doppler can be used to detect areas of angiogenesis in testicular tumors.