ICIVET-LITORAL   24728
INSTITUTO DE CIENCIAS VETERINARIAS DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Novel Findings in Crocodilian Immunology
Autor/es:
NAZAR, F. N.; MOLEÓN, MARÍA S.; SIROSKI, PABLO A.; ORTEGA, HUGO H.
Lugar:
Skukuza
Reunión:
Congreso; 24rd Working Meeting of the IUSN-SSC. Crocodile Specialist Group; 2016
Resumen:
Researches on crocodilian immunology have demonstrated interesting findings and promising perspectives. In recent years, the study of a significant number of genes coding for immune molecules have been identified in ectothermic vertebrates, and most of them tending to be cloned. Cytokines are a family of secreted and regulatory molecules with a paracrine or autocrine hormone-like activity required to develop a immune response. They join to the surface of the cell receptors with high affinity and trigger a set of protein synthesis, playing an important role in immune cell differentiation and activation that depends on the producing and responding cell type, and on the phase of the immune response. Interleukins are a large group of citokynes. Composition and bioactivity of these immunomodulatory proteins has been studied in many species but not in crocodilians. So, a detailed analysis on the identification and characterization of cytokines is very useful to understand the mechanism involved in the defense of these species. Also, they can be used as bioindicators to evaluate the individual and environmental health. In order to detect cytokines, mRNA samples from spleen tissues of Caiman latirostris were obtained. We tried sets of primers designed from alignments of phylogenetically related species sequences, and others reported for avian and fish species. The expression of proinflamatories and antinflamatories cytokines (interleukin [IL] 1β and IL 4, respectively) were detected by reverse transcription PCR (RT-PCR) and real time PCR. These findings open a new window in the knowledge of the crocodilian immunology with the aims to identify more proteins of this group, and how they exert their activities in the organism and ecological context.