Alterations of adiponectin receptors and 5-AMP kinase expression in follicular structures of cows with cystic ovarian disease

DIAZ, PU; ORTEGA HH; SALVETTI NR; HEIN G; GAREIS N; PANZANI, CG; REY F

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXIV Reunión anual de la SAI; XLVIII Reunión anual de la SAFE; VII Reunión anual de la NANOMEDAR; 2016

Adiponectin is an adipokine produced by adipose tissuewhich regulates several reproductive processes. Its role insteroidogenesis, considering the energy balance for the ovaryfunctions, could be potentially associated to the development ofreproductive disorders such as cystic ovarian disease (COD).The aim of this study was to determine the expression of componentsof the adiponectin system in ovarian follicles of cowswith spontaneous COD and control cows. Thus, the receptorsof adiponectin, AdipoR1 and AdipoR2, and the enzyme 5-AMPkinase (AMPK) were evaluated. Animals with spontaneous COD(n=8) and control cows (n=8) were ovariectomized and ovariesobtained were processed histologically to embedded in paraffin.Expression of target protein was assessed by indirect immunohistochemistryon ovarian tissue sections. Results showedsimilar expression of AdipoR1 in follicular structures evaluatedin ovaries from control cows and cows with COD, showing atendency to decrease the expression in granulosa cells of cystsregarding to antral follicles (as reference structure) of controlcows (p<0.1). However, a higher expression of AdipoR2 wasdetected in theca cells of cysts compared to antral follicles ofcontrol cows (p<0.05) without differences in granulosa cells.Moreover, the expression of AMPK in theca cells of cysts washigher compared to control antral follicles (p<0.05) without differencesin granulosa cells of the follicles analyzed. The resultsof the present study showed variations in proteins involved inresponse and signaling to adiponectin. Considering the associationof this adipokine with several metabolic functions, such asimproving the insulin sensitivity, and also its essential role forfollicular growth and maturation, the alterations here detectedcould indicate a potential contribution of this cytokine in ova rianfunction related to reproductive disorders.