Use of ultrasonography in preclinical studies: tumor measurements using B-mode ultrasound real time, and vascular characterization by Power Doppler ultrasound of tumors in immunodeficient mice

DIAZ, A; SALVETTI NR; ORTEGA HH; STASSI, A; MARELLI B; BELOTTI M; SALINAS, F; PODESTA EJ

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXIV Reunión anual de la SAI; XLVIII Reunión anual de la SAFE; VII Reunión anual de la NANOMEDAR; 2016

Sociedad Argentina de Investigación Clínica (SAIC).

The standard method formeasurement of subcutaneous tumors in immunodeficient mice is through the useof calipers. Nevertheless, it introduces a lot of bias. At the same time, in the last decades, the use of ultrasonography as adiagnosis method for tumors has advanced, including the possibility of gettingbetter assessment to gauge those neoplasms of many different ways. Thevasculature of a tumor is characterized by poor vascular function, variableblood flow, leaky and immature blood vessels, and a high degree of tortuosityand irregularity.  Ultrasoundpower Doppler image is widely applied for observing the perfusion ofvasculature because it provides the noninvasive and reproducible informationand helps in assessing longitudinally the angiogenesis-related disease as wellas monitoring the therapeutic response. The objective of this study is toreport a different option to evaluate tumors in preclinical procedures. NUDEmice injected with subcutaneous breast neoplasm, on the right flank, wereevaluated every week during 2 months, beginning at days of the injection ofneoplastic cells; they were assessed by ultrasound with a color Dopplerequipment (Z6 Vet, Mindray, China), coupled to a linear probe a 5.0-10 MHz (75L50EAV, Mindray). For differentmensuration of the tumors, B-modereal time scanning was used . Later, the equipment was setting to ?powerDoppler? function to evaluation of blood flow intensity in neoplasm region. Thevalues obtained were: conjugated diameter, volume and blood flow intensity ofthe tumor at different moments in the protocol. These parameters allows us toget more measures than caliper mensuration, in less time of manipulation of theanimals (the acquisition duration of each power Doppler image was about 8?10 sin this study), with a non-invasive method, providing more objective data. Theblood flow rate in the microcirculation associated with angiogenesis plays animportant role in the progression and treatment of cance