Expression of receptors for TGF-B Superfamiliy in cows with Spontaneous and ACTH-Induced Cystic Ovarian Disease.

HEIN G.; BERTOLI, J; SALVETTI N.R.; MATILLER V; RODRIGUEZ F.; AMWEG, A.; REY F.; QUERCIA, E

Mar del Plata

Congreso; LXI Reunión Científica anual de la Sociedad Argentina de Investigación Clínica(SAIC)-LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI). XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental (SAFE). Reunión Asociación; 2016

Sociedad Argentina de Investigación Clínica (SAIC).

Expression of receptors for TGF-B Superfamily in cows with spontaneous and ACTH-induced cystic ovarian disease. Valentina Matiller 1,2, Gustavo Hein 1, Amweg AN 1, Rodriguez FM 1, Bertoli José 2, Ernesto Quercia 3, Florencia Rey 1 and Natalia R Salvetti 1.(1) Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL) / Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina.(2)Cátedra de Producción de Bovinos de Leche. Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina.3. Profesional. Actividad Privada.Preliminary results shows significant changes in the expression of components of the TGF-B isoforms TGF-B1, 2 and 3, and inhibin / activin / follistatin system in ovarian structures of dairy cattle with spontaneous and ACTH-induced cystic ovarian disease (COD). Given the evidence of its role as an important molecules in paracrine and autocrine signaling pathways that regulate the growth of ovarian follicle, and knowing that this components carry out their actions through binding to two types of membrane receptors, the objective of the present work was examine the expression of TGF-B receptor (TGF-BR) I, TGF-BRII, TGF-BRIII, Activin Receptor (ACVR)IB and ACVRIIB in ovaries of cows with COD previously evaluated for their related ligands. We worked with sections obtained from bovine ovaries with spontaneous COD (n=10), ACTH-induced COD (n=10), and controls ovaries (n=10). In 4 um thick sections, the presence and expression of receptors was determined by indirect immunohistochemistry in granulosa and theca cells. Type I receptors expressed higher staining (p