Micronucleus (MN) and other nuclear abnormalities (NAs) in Caiman latirostris (Broad-snouted caiman) after in ovo exposure to pesticide formulations and mixtures through incubation material

LARRIERA ALEJANDRO; SIROSKI, PABLO A.; LÓPEZ GONZÁLEZ EVELYN C.; POLETTA GISELA L.

Montevideo

Congreso; X Congreso Internacional de la Asociación Latinoamericana, Mutagénesis, Carcinogénesis y Teratogénesis Ambiental (ALAMCTA). II Congreso de la Sociedad Uruguaya de Radioprotección. I Jornada de Salud y Toxicología Ambiental.; 2016

Asociación Latinoamericana, Mutagénesis, Carcinogénesis y Teratogénesis Ambiental (ALAMCTA).

The aim of this study was to evaluate the genotoxicity induced by Chlorpyrifos- (CPF) (Lorsban 48E® -48% p.a.), Cypermethrin (Atanor® -CYP, 25% p.a.) and Glyphosate (Roundup Full II® -GLY, 66.2% p.a.) based formulations and the complex mixture (M= CPF + CYP + GLY) in Broad-snouted caiman hatchlings, after in ovo exposure through incubation material.Concentrations equivalent to those recommended in agricultural practices for application in soybean crops (1) and a half of them (1/2) were sprayed upon incubation material at the beginning of the incubation period (under controlled conditions of temperature and humidity).Eggs (N= 108) harvested from three different nests in the Natural Managed Reserve ??El Fisco?? (30° 11? 26?? S; 61° 0?27??W; Dpto. San Cristóbal, Santa Fe, Argentina), under the ?Proyecto Yacaré? ranching program activities were used. They were randomly distributed into 9 experimental groups (12 eggs per experimental group, two replicates of 6 eggs each): a negative control (NC) sprayed with tap water (3,5 ml); two groups sprayed with CPF (CPF1 0.8% and CPF1/2 0.4% ); two sprayed with CYP (CYP1 0.15% and CYP1/2 0.075%); two sprayed with GLY (GLY1 2% and GLY1/2 1%); and M sprayed with the three formulations at the recommended concentrations (M1) and the half (M1/2). At time of hatching, blood samples were obtained from each neonate for the determination of the frequency of Micronucleus (FMN) and other nuclear abnormalities (NAs) on erythrocytes.A significant increase in the FMN was observed in CYP1/2, GLY1, GLY1/2, M1 and M1/2. Regarding other NAs, we only observed differences in the number of binucleated cells in GLY1/2 and M1, compared with NC, respectively (p