Micronucleus test and nuclear abnormalities in Caiman latirostris (Broad-snouted caiman) exposed in vivo to commercial formulations of insecticides: endosulfan and cypermethrin

E C LOPEZ GONZALEZ; ALEJANDRO LARRIERA; PABLO A. SIROSKI; GISELA L. POLETTA

Lake Charles, Louisiana.

Congreso; 23rd Working Meeting of the Crocodile Specialist Group; 2014

The agricultural frontier expansion in Argentina led to a high transformation of many environments occupied by natural populations of Caiman latirostris. The aim of this study was to evaluate genotoxicity induced by insecticides Endosulfan (END) and Cypermethrin (CYP) on C. latirostris hatchlings, using the Micronuclei (MN) and other nuclear abnormalities (NA) as biomarkers. C. latirostris hatchlings, 20 days old, were exposed to two concentrations of END (END1 and END2), and two of CYP (CYP1 and CYP2), including those recommended for their application in crops, using ethanol as a vehicle and a control without treatment (NC). Animals were maintained in plastics pens, during two months and concentration of insecticides progressively decreased through time in order to simulate their degradation in water in natural conditions. After exposure, blood samples were taken to all animals for determination of the Frequency of MN (FMN) and NA (FNA; vacuolated nuclei, notched nuclei, buds, binuclei, eccentric nuclei) in erythrocytes as markers of genotoxicity. The NC and vehicle control showed no difference in the FMN indicating that ethanol caused no genotoxic damage (p>0.05). Statistical significant differences were found in the FMN between exposed groups: CIP2 (p=0,023) and END1 (p