ICIVET-LITORAL   24728
INSTITUTO DE CIENCIAS VETERINARIAS DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Expression of il-6 and il1-a cytokines, in ovarian follicles of cows with cystic ovarian disease (COD).
Autor/es:
BARAVALLE M.E; STASSI A; VELÁZQUEZ MML; BELOTTI M; DIAZ P; PERALTA B; SALVETTI NR; ORTEGA, H.H.
Lugar:
Montevideo
Reunión:
Otro; Curso Internacional Flow Cytometry and Cell Sorting in Biotechnology and Biomedicine Research; 2014
Institución organizadora:
Institute Pasteur-Montevideo
Resumen:
Ovulation is currently considered an acute and local inflammatory process, and cytokines have fundamentals roles because they influence the production of several mediators. Considering the multiple factors involved in these processes (ovulation / inflammation), the alteration of one or more components may contribute to the pathogenesis of reproductive diseases as cystic ovarian disease (COD), one of the most common reproductive disorders in dairy cattle. This disease causes considerable economic losses for livestock. Several studies show evidence that cytokines not only act in regulating immune responses but also other physiological processes such as ovulation. In the ovary, cytokines are secreted by immune cells as well as theca and granulosa cells. The aim of this project is to evaluate the expression levels of IL-6 and IL1-a cytokines, in ovaries of animals with spontaneous COD and controls in proestrus. Increased protein expression levels of IL1-a and IL-6 were observed in granulosa cells from cyst compared with antral follicles, used as controls, without change in the theca cells. These results differed from that obtained at the level of gene expression, and may be due to post-translational modification performed in the different cell populations. The results obtained in this study suggest that an alteration in mechanisms associated with the inflammatory process, and these interleukins as factors involved in this process, could be part of the pathogenesis of cystic ovarian disease. Further studies will involved the presence of these cytokines in follicular fluid from normal and cystic follicles, by Flow Cytometry. Probably one of the tools applied by this methodology, allow us to evaluate and to quantify a large number of cytokines simultaneously