ICIVET-LITORAL   24728
INSTITUTO DE CIENCIAS VETERINARIAS DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
MACROCAPSULES FOR MAINTENANCE OF PROBIOTIC VIABILITY DURING STORAGE FOR YOUNG CALVES
Autor/es:
ZIMMERMANN, JORGE ALBERTO; ASTESANA, DIEGO; BERISVIL, AYELÉN PATRICIA; FUSARI, MARCIA; GELOTTI, NICOLÁS; MARTÍ, LUIS; SOTO, LORENA
Lugar:
San Miguel de Tucumán
Reunión:
Simposio; IV International Symposium on Lactic Acid Bacteria.; 2013
Institución organizadora:
Centro de Referencia para Lactobacilos (CERELA-CONICET)
Resumen:
Periodic administration of a probiotic inoculum of bovine origin may favor establishment of a stable and balanced intestinal microbiota, which would improve the health of the calves. The viability and number of microorganisms inoculated is vital because the suggested minimum level (SML) of bacteria to produce beneficial effects is 106 CFU/ml. A technique that is currently being implemented to maintain the viability of probiotics is encapsulation. The aim of this study was to evaluate the viability of a co-culture of Lactobacillus casei DSPV 318T and L. plantarum DSPV 354T macroencapsulated through time in different store temperatures. Both strains were grown in pasteurized whey (PW) together over night. Then, this culture was mixed with alginate 1% w/v (final concentration). This mixture was disposed in 1 ml molds and frozen during 3 h. Thereafter the capsules were suspended for 1 h in CaCl2 solution (0.1 M) for polymerization of alginate. Subsequently, half of the capsules were placed in a chitosan solution (0.4% w/v) for 40 min to create an outer coating of the capsules. Then, both the coated capsules as uncoated were incubated in PW for 9 h at 37 °C to increase cell counts. Macrocapsules were stored at room temperature (28 °C) and refrigeration (4 °C). For determination of viability, the capsules were dissolved in sodium citrate solution (1% w/v). Serial decimal dilutions were made and spread on MRS agar to determine the total counts and on Lactobacillus plantarum selective medium (LPSM) for L. plantarum DSPV 354T counts. For determination of L. casei DSPV 318T counts, LPSM counts were subtracted from the total counts made ​​in MRS. Cell viability was determined weekly for a period of 6 weeks Determinations were in triplicate. Results were analyzed using ANOVA and Tuckey?s test. Co-culture (total counts) viability was higher (P < 0.001) for coated macrocapsules, in refrigeration storage (P < 0.001), and the best (P = 0.032) interaction between factors was coated capsule + refrigeration, having 8.5 log CFU/g counts at the end of the experiment. L. plantarum DSPV 354T viability was higher (P < 0.001) for coated macrocapsules, in refrigeration storage (P = 0.007), and the best (P < 0.001) interaction between factors was: coated capsule + refrigeration, obtaining counts of 7 log CFU/g at the end of the experiment. L. casei DSPV 318T viability was higher (P < 0.001) for coated macrocapsules, in refrigeration storage (P<0.001), and the best (P = 0.876) interaction between factors was: coated capsule + refrigeration, obtaining counts of 8.5 log CFU/g counts at the end of the experiment. Probiotic?s encapsulation in macrocapsules coated with chitosan and storing them in refrigeration, ensures maintenance of adequate viability to exert a probiotic effect for at least 6 weeks.