Biomarkers of geno- and cytotoxicity in the native broad-snouted caiman (Caiman latirostris): chromosomal aberrations and mitotic index

CHACÓN CAMILA FELISA; GISELA LAURA POLETTA; EVELYN CECILIA LÓPEZ GONZÁLES

MUTATION RESEARCH. GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2021 vol. 867

1383-5718

We evaluated the sensitivity of the chromosomal aberration (CA) and mitotic index (MI)assays on peripheral blood lymphocytes (PBLs) of Caiman latirostris, following exvivo exposure to the alkylating agent, MMS. Two concentrations of MMS were testedin cultured peripheral blood. Relative to controls, MMS exposure reduced the numberof metaphases observed, but both the numbers of cells with MN and the percentagesof aberrant metaphases increased. The types of CA identified were chromosome andchromatid breaks, chromosomal rearrangements, monosomies, and nullisomies, withsignificantly higher values in the MMS-exposed groups. The incorporation of the MIand CA tests in C. latirostris can provide information on damage caused by xenobioticexposures