ICIVET-LITORAL 24728
INSTITUTO DE CIENCIAS VETERINARIAS DEL LITORAL
Unidad Ejecutora - UE
artículos
Título:
Relative expression of genes associated with adhesion to bovine mammary epithelial cells by Streptococcus uberis
Autor/es:
RENNA, MARÍA SOL; FESSIA, ALUMINÉ SOLEDAD; RASPANTI, CLAUDIA G.; FESSIA, ALUMINÉ SOLEDAD; RASPANTI, CLAUDIA G.; DIESER, SILVANA A.; ODIERNO, LILIANA M.; DIESER, SILVANA A.; ODIERNO, LILIANA M.; RENNA, MARÍA SOL
Revista:
RESEARCH IN VETERINARY SCIENCE
Editorial:
ELSEVIER SCI LTD
Referencias:
Año: 2020 vol. 132 p. 33 - 41
ISSN:
0034-5288
Resumen:
Streptococcus uberis is one of the most prevalent environmental pathogens of bovine mastitis. Biofilm growthability by S. uberis looks to depend first upon the adherence of cells to a surface. The S. uberis ability to adhere tomammary gland epithelia might provide an advantage to colonize the lactating mammary gland. The objectivesof this study were (a) to select S.uberis strains according to their ability to form biofilm, (b) to determine adherenceto and internalization into MAC-T cells and (c) to investigate the expression profile adherence genes inthese S. uberis strains. For the assays, the MAC-T bovine mammary epithelial cell line was used. Relative expressionof genes acdA, lmb, scpA, sua, fbp and lbp was quantified by RT-qPCR. We observed that the RC38 strainfrom clinical bovine mastitis showed in the six genes higher values than control in both conditions. While thestrain with greater ability to adhere, from clinical mastitis and biofilm producer (RC29) evidenced higher valuesin group 1 (G1) (bacteria after the initial contact with MAC-T cells) and decrease in group 2 (G2) (both adheredand internalized bacteria) than control. Strains with a moderate or strong capacity for biofilm productionshowed significantly lower relative expression values in the G2. In all adherence associated genes, strain RC19showed relative expression values incremented in G1, while in G2 decreased expression. In conclusion, we did notfind a single profile of relative expression because the relative expression levels of each gene differed dependingon the strain and the co-culture stage of S. uberis cells from which RNA was obtained.
