Impaired insulin signaling pathways affect ovarian steroidogenesis in cows with COD

GAREIS, NC (EQUAL CONTRIBUTOR); RODRÍGUEZ, FM; ORTEGA, H.H.; HUBER, E (EQUAL CONTRIBUTOR); SALVETTI, N.R.; REY, F.; HEIN, GJ; ANGELI, E.; GAREIS, NC (EQUAL CONTRIBUTOR); RODRÍGUEZ, FM; ORTEGA, H.H.; HUBER, E (EQUAL CONTRIBUTOR); SALVETTI, N.R.; REY, F.; HEIN, GJ; ANGELI, E.

ANIMAL REPRODUCTION SCIENCE

ELSEVIER SCIENCE BV

Año: 2018 p. 298 - 312

0378-4320

Cystic ovarian disease (COD) represents an important cause of infertility in dairy cattle and is associated with multiple physiological disorders. Steroidogenesis, which is necessary to ensure normal ovarian functions, involves multiple enzymatic pathways coordinated by insulin and other proteins. We have previously shown that cows with COD have an altered insulin response. Therefore, in the present study, we evaluated further alterations in intermediates downstream of the PI3K pathway and pathways mediated by ERK as critical signals for the expression of steroidogenic enzymes in the ovaries of control cows and cows with spontaneous COD. To this end, we evaluated the gene and protein expression of pan-AKT, mTOR, ERK1/2, and steroidogenic enzymes by real-time PCR and immunohistochemistry. Steroid hormone concentrations were assessed at systemic and intrafollicular level. Results showed altered expression of intermediate molecules of the insulin signaling pathway, whose action might modify the synthetic pathway of steroidogenic hormones. Similarly, the expression of steroidogenic enzymes and the concentration of progesterone in serum and follicular fluid were altered. These alterations support the hypothesis that systemic factors contribute to the development and/or maintenance of COD, and that metabolic hormones within follicles such as insulin exert determinant effects on ovarian functionality in cows with COD.