Altered expression of cytokines IL-1α, IL-6, IL-8 and TNF-α in bovine follicular persistence

STASSI, A.F.; BARAVALLE, M.E.; BELOTTI, E.M.; REY, F.; GAREIS, N.C.; DÍAZ, P.U.; RODRÍGUEZ, F.M.; LEIVA, C.J.; ORTEGA, H.H.; SALVETTI, N.R.; STASSI, A.F.; BARAVALLE, M.E.; BELOTTI, E.M.; REY, F.; GAREIS, N.C.; DÍAZ, P.U.; RODRÍGUEZ, F.M.; LEIVA, C.J.; ORTEGA, H.H.; SALVETTI, N.R.

THERIOGENOLOGY

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2017 vol. 97 p. 104 - 112

0093-691X

In dairy cattle, cystic ovarian disease (COD) is an important cause of subfertility, and two of the main signs are ovulation failure and follicular persistence. The aim of this study was to examine the expression of the cytokines IL-1α, IL-6, IL-8 and TNF-α in ovarian follicular structures at different times of persistence in a model of follicular persistence induced by prolonged treatment with progesterone in dairy cows. Protein expression of IL-1α, IL-6, IL-8 and TNF-α was evaluated by immunohistochemistry. Additionally, IL-6 concentration in follicular fluid and serum was determined by ELISA. IL-1α, IL-6, IL-8 and TNF-α expression was increased in follicles with different persistence times in relation to the control dominant follicles, in granulosa cells. For IL-6, IL-8 and TNF-α, this increase was detected early (P0: expected time of ovulation and/or P5: 5 days of follicular persistence). Additionally, theca cells showed an increase in IL-6 in antral (groups P10 and P15) and persistent follicles (group P10) related to dominant follicles from the control group (p