Probiotic effect on calves infected with Salmonella Dublin: haematological parameters and serum biochemical profile

ZBRUN, M.V.; BERISVIL, A.; FRIZZO, L.S.; SOTO, L. P.; BLAJMAN, J.E.; ROSMINI, M.R.; ASTESANA, D.M.; SALVETTI, N; SIGNORINI, M.L.

Beneficial Microbes

Wageningen Academic Publisher

Año: 2016 vol. 7 p. 23 - 33

1876-2883

The aim of this study was to evaluate the effect of a probiotic/lactoseinoculum on haematological and immunological parameters and renal and hepaticbiochemical profiles before and during a SalmonellaDublin DSPV 595T challenge in young calves. Twentyeight calves, divided into control group(CG) and probiotic group (PG) were used. The PG was supplemented with 100 glactose/calf/d and 1010 CFU/calf/d of each strain of a probioticinoculum composed of Lactobacillus caseiDSPV318T, Lactobacillus salivarius DSPV315Tand Pediococcus acidilactici DSPV006Tthroughout the experiment. The pathogen was administered on day 11 of theexperiment, at an oral dose of 109 CFU/animal (LD50).Aspartate aminotransferase (AST), gamma glutamyl transpeptidase (GGT), urea, redblood cells (RBC), haemoglobin, haematocrit, mean cell haemoglobin (MCH), meancorpuscular volume (MCV), mean corpuscular haemoglobin concentration (MCHC),white blood cells (WBC), lymphocytes, neutrophils, band neutrophils, monocytes,eosinophils, basophils and the lymphocytes/neutrophils relationship weremeasured on days 1, 10, 20 and 27 of the experiment. In addition, animals werenecropsied to evaluate IgA production in the jejunal mucosa. The mostsignificant differences caused by the administration of the inoculum/lactosewere found during the acute phase of Salmonellachallenge (9 days after challenge), when a difference between groups in neutrophils/lymphocytesrelationship were detected. These results suggest that the probiotic/lactoseinoculum administration increases the calf?s ability to respond to the diseaseincreasing the systemic immune response specific. No differences were found inhaemoglobin, haematocrit, MCH, MCHC, AST, urea, GGT, band neutrophils,eosinophils, monocytes and IgA in the jejunum between the two groups of calvesunder the experimental conditions of this study. Further studies must beconducted to evaluate different probiotic/pathogens doses and differentsampling times, to achieve a greater understanding of the effects of thisinoculum on intestinal infections in young calves and of its mechanism ofaction.