ICIVET-LITORAL   24728
INSTITUTO DE CIENCIAS VETERINARIAS DEL LITORAL
Unidad Ejecutora - UE
artículos
Título:
Cytoskeletal proteins in the follicular wall of normal and cystic ovaries of sows
Autor/es:
SANT´ANA F; REIS JUNIOR, JL; ARAUJO RLS; GIMENO EJ; ORTEGA HH
Revista:
PESQUISA VETERINáRIA BRASILEIRA
Editorial:
REVISTA PESQUISA VETERINARIA BRASILEIRA
Referencias:
Año: 2015 vol. 35 p. 119 - 124
ISSN:
0100-736X
Resumen:
The expression of cytoskeletal proteins was evaluated immunohistochemically in 36normal ovaries sampled from 18 sows and 44 cystic ovaries sampled from of 22 sows, wasevaluated. All sows had history of reproductive problems, such as infertility or subfertility.The immunohistochemically stained area (IHCSA) was quantified through image analysisto evaluate the expression of these proteins in the follicular wall of secondary, tertiary, andcystic follicles. Cytokeratins (CK) immunoreactivity was strong in the granulosa cell layer(GC) and mild in the theca interna (TI) and externa (TE) of the normal follicles. There wassevere reduction of the reaction to CK in the GC in the cystic follicles, mainly in the luteinizedcysts. The immunoreactivity for vimentin was higher in the GC from normal and cysticfollicles in contrast with the other follicular structures. In the luteinized cysts, the IHCSAfor vimentin was significantly higher in TI and in both observed cysts, the labeling wasmore accentuated in TE. Immunohistochemical detection of desmin and α-SMA was restrictedto the TE, without differences between the normal and cystic follicles. The resultsof the current study show that the development of ovarian cysts in sows is associated tochanges in the expression of the cytoskeletal proteins CK and vimentin.