ICIVET-LITORAL 24728
INSTITUTO DE CIENCIAS VETERINARIAS DEL LITORAL
Unidad Ejecutora - UE
artículos
Título:
In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli
Autor/es:
SIROSKI, PA; RUSSI, N; ORTEGA HH; FORMENTINI, EA
Revista:
RESEARCH IN VETERINARY SCIENCE
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2015 vol. 98 p. 98 - 105
ISSN:
0034-5288
Resumen:
The in vitro synergistic activity between ciprofloxacin and serum of broad snouted caiman on Escherichiacoli was studied. The estimated MIC value of ciprofloxacin was 0.0188 μg/ml, and two assays of killcurve during 5 hours were performed: the first one in a standard culture medium and the second onein the presence of caiman serum. Different concentrations of ciprofloxacinwere tested. Ciprofloxacin showedhigher values of bacterial elimination rate in the presence of caiman serum in all concentrations tested.The combined activity of sub-inhibitory concentrations of ciprofloxacin and the humoral immune factorspresent in caiman serum determined an increase in the bacterial elimination observed in this assay.Wesuggest that the antibacterial activity of complement and natural antibodies present in caiman serum,which can bind to both Gram-negative and Gram-positive bacteria and acting through the classical complementpathway, can inhibit bacterial growth of Escherichia coli by lysis.
