ICIVET-LITORAL   24728
INSTITUTO DE CIENCIAS VETERINARIAS DEL LITORAL
Unidad Ejecutora - UE
artículos
Título:
Intermediate filament proteins expression and carbohydrate moieties in trophoblast and decidual cells of mature cat placenta
Autor/es:
FERNÁNDEZ PE; DIESSLER ME; PACHAMÉ A; ORTEGA, H.H; GIMENO EJ; PORTIANSKY EL; BARBEITO CG
Revista:
REPRODUCTION IN DOMESTIC ANIMALS (1990)
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2014 vol. 49 p. 263 - 269
ISSN:
0936-6768
Resumen:
The aim
of this study was to characterize cytoskeletal intermediate filament proteins
and glycoconjugates of syncytiumtrophoblast, cytotrophoblast and decidual cells
of feline endotheliochorial placenta. Samples from 12 normal pregnant female
cats, after 45 5 days of gestation, were
obtained removing the uterine horns by hysterectomy. Sections were processed
for routine observation and for immunohistochemistry using anticytokeratin,
antivimentin and antidesmin antibodies. In addition, lectin histochemistry was
performed using a panel of several biotinylated lectins to characterize
glycosides expression profile. Cytotrophoblast and syncytiotrophoblast showed
immunoreactivity only with acidic and basic cytokeratins. Decidual cells were
only positive to vimentin, consistent with their origin from endometrial
fibroblasts. Trophoblast expressed a broad population of glycans, highly exposing
terminal N-acetyl glucosamine residues and non-sialylated galactose and
N-acetyl galactosamine oligomers. Oligosaccharides bound by Phaseolus vulgaris
erythroagglutinin were the only highly branched N-linked residues evidenced in
cats, and they were restricted to the syncytium. Unlike results reported on
humans, mice and rats on lectin affinity of decidual cells, sialid acids and
complex N-linked oligosaccharides were not demonstrated in cats. Glycosylation
of proteins determines many of their final properties, thus becoming essential
for the embryo-maternal dialogue during implantation and placentation. Changes
in glycosylation pattern have been related to pathological pregnancies in other
species. Hence, the knowledge about glycosylation profile of the normal cat
placenta may lead to a better understanding of both normal and pathological
reproductive events.