Staphylococcus aureus chronic intramammary infection modifies the expression of transforming growth factor beta (TGF-β) subfamily components during active involution.

ANDREOTTI C. S.; PEREYRA E.A.L.; BARAVALLE C.; RENNA, M.S.; ORTEGA H.H.; CALVINHO L.F.; DALLARD B.E.

RESEARCH IN VETERINARY SCIENCE

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2014 vol. 96 p. 5 - 14

0034-5288

The objectives of this study were to determine whether S. aureus chronic intramammary infection (IMI) influences protein expression of TGF-â subfamily components and collagen I and to examine the histomorphometric changes that occur in mammary stroma and parenchyma during active mammary gland involution. Twenty-one Holstein non-pregnant cows in late lactation either uninfected or with chronic natural S. aureus IMI were included in this study. Cows were slaughtered at 7, 14 and 21 d after cessation of milking and samples for immunohistochemical and morphometric analysis were taken. Protein expression of TGF-â1, TGF-â2 and TGF-â3 was significantly higher in chronically infected quarters than in uninfected controls at the three involution stages studied. Immunostaining of TGF-âR1 and TGF-âR3 and collagen I was significantly higher in S. aureus-infected quarters than in uninfected controls at every involution time evaluated. The percentages of tissue area composed of parenchyma and intralobular stroma were significantly higher in S. aureus-infected than in uninfected quarters. Chronic S. aureus mastitis modifies protein expression of the three TGF-â isoforms and type 1 and 3 receptors, which was associated with changes directed to limit the scope of inflammation and injury to the host.