ICIVET-LITORAL   24728
INSTITUTO DE CIENCIAS VETERINARIAS DEL LITORAL
Unidad Ejecutora - UE
artículos
Título:
Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovarian disease
Autor/es:
BARBEITO, C.G.; ORTEGA H.H.; MATILLER V.; GIMENO, E.J.; SALVETTI N.R.
Revista:
REPRODUCTION IN DOMESTIC ANIMALS (1990)
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2013 vol. 48 p. 850 - 857
ISSN:
0936-6768
Resumen:
Numerous experimental models in different species have been developed for the study
24 of polycystic ovarian syndrome. In the present study we used a model of induction ofof polycystic ovarian syndrome. In the present study we used a model of induction of
25 polycystic ovaries in rats by exposure to constant light to study the distribution andpolycystic ovaries in rats by exposure to constant light to study the distribution and
26 variations of glycosylated residues present in the different ovarian structures. Sevenvariations of glycosylated residues present in the different ovarian structures. Seven
27 biotinylated lectins were used (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) onbiotinylated lectins were used (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) on
28 tissue sections and detection was performed using the streptavidine/peroxidase method.tissue sections and detection was performed using the streptavidine/peroxidase method.
29 In tissue sections was observed an increase in affinity for Con-A in the granulosa andIn tissue sections was observed an increase in affinity for Con-A in the granulosa and
30 theca interna of growing follicles and cysts in animals with polycystic ovaries intheca interna of growing follicles and cysts in animals with polycystic ovaries in
31 relation to the control group. Follicular cysts showed higher affinity for WGA andrelation to the control group. Follicular cysts showed higher affinity for WGA and
32 RCA-I which growing follicles in the same group and there was a decrease in affinityRCA-I which growing follicles in the same group and there was a decrease in affinity
33 for PNA in the cysts in relation to the growth of follicles in both groups. Atretic folliclesfor PNA in the cysts in relation to the growth of follicles in both groups. Atretic follicles
34 in both groups showed greater labeling with lectins PNA, SBA and RCA-I in relation toin both groups showed greater labeling with lectins PNA, SBA and RCA-I in relation to
35 healthy follicles. It could also be noted that the zona pellucida of cystic follicles lost thehealthy follicles. It could also be noted that the zona pellucida of cystic follicles lost the
36 affinity for the lectin Con-A. There was no staining on follicles in any category with theaffinity for the lectin Con-A. There was no staining on follicles in any category with the
37 lectins DBA and UEA-I, although it was staining in the corpus luteum (control group)lectins DBA and UEA-I, although it was staining in the corpus luteum (control group)
38 and in the mesothelium and interstitial glands of both groups with DBA. Theseand in the mesothelium and interstitial glands of both groups with DBA. These
39 observations probably reflect changes in the glycosaminoglycans present in the differentobservations probably reflect changes in the glycosaminoglycans present in the different
40 ovarian compartments or in the glycosylation of cellular components essential forovarian compartments or in the glycosylation of cellular components essential for
41 proper follicular dynamics.proper follicular dynamics.