CIMEC   24726
CENTRO DE INVESTIGACION DE METODOS COMPUTACIONALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Hyphenation of 2D heart cutting CIEF/CE-MS
Autor/es:
SYDES, DANIEL; KLER, PABLO A.; HUHN, CAROLIN
Lugar:
GRAZ
Reunión:
Congreso; ANAKON 2015; 2015
Resumen:
The hyphenation of capillary isoelectric focusing (CIEF) with mass spectrometry (MS)is a very promising tool for the characterization of proteins and peptides especially for R&D and quality control of monoclonal antibodies. However, the CIEF-MS coupling is still challenging due to the high number of carrier ampholytes that hinder MS detection. We seek to establish a 2D separation approach for CIEF/CE-MS to separate analytes from carrier ampholytes in the second dimension (CE) prior to MS detection. A microfluidic chip with a common intersection for both separation dimensions is used as interface for hyphenation. First 2D experiments using myoglobin as a model analyte show that the CIEF pH gradient can be sampled sequentially (heart cutting) and each fraction injected via the microfluidic chip interface into the second CE separation dimension and identified via MS. Positioning of the pH gradient within the setup was realized via different plug lengths of anolyte and catholyte, respectively.