ITA-NOA   24624
INSTITUTO DE TECNOLOGIA AGROINDUSTRIAL DEL NOROESTE ARGENTINO
Unidad Ejecutora - UE
artículos
Título:
Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy- associated vacuolar processes.
Autor/es:
MARANO, M. R.; FILIPPONE, M.P.; ALEMANO, S.; ROESCHLIN, R.A.; GMITTER JR.F.G.; VOJNOV, A.A.; FAVARO, M.A.; GADEA, J.; CASTAGNARO, A.P.; CHIESA, M.A.; MARANO, M. R.; FILIPPONE, M.P.; ALEMANO, S.; ROESCHLIN, R.A.; GMITTER JR.F.G.; VOJNOV, A.A.; FAVARO, M.A.; GADEA, J.; CASTAGNARO, A.P.; CHIESA, M.A.
Revista:
MOLECULAR PLANT PATHOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2017 vol. 18 p. 1267 - 1281
ISSN:
1464-6722
Resumen:
Xanthomonas citri ssp. citri (X. citri) is the causal agent of Asiatic citrus canker, a disease that seriously affects most commercially important Citrus species worldwide. We have previously identified a natural variant, X. citri AT, that triggers a host-specific defense response in Citrus limon. However, the mechanisms involved in this canker disease resistance are unknown. In this work, the defense response induced by X. citri AT was assessed by transcriptomic, physiological and ultrastructural analyses and the effects on bacterial biofilm formation were monitored in parallel. We show that X. citri AT triggers a hypersensitive response associated with the interference on biofilm development and arrest of bacterial growth in C. limon. This plant response involves an extensive transcriptional reprogramming setting in motion cell wall reinforcement, oxidative burst and accumulation of salicylic acid (SA) and phenolic compounds. Ultrastructural analyses revealed subcellular changes involving the activation of autophagy-associated vacuolar processes. Our findings show the activation of SA-dependent defense in response to X. citri AT and suggest a coordinated regulation between SA and flavonoids pathways, which is associated with autophagy mechanisms that control pathogen invasion in C. limon. Furthermore, this defense response protects C. limon plants from disease upon subsequent challenges by pathogenic X. citri. This knowledge will allow to rationally exploit the plant immune system as a biotechnological approach to manage the disease.