INVESTIGADORES
BLANCO Flavio Antonio
artículos
Título:
Annotation, Phylogeny and Expression Analysis of the Nuclear Factor Y Gene Families in Common Bean (Phaseolus vulgaris)
Autor/es:
RÍPODAS, CAROLINA; CASTAINGTS, MELISSE; CLÚA, JOAQUÍN; BLANCO, FLAVIO; ZANETTI, MARÍA EUGENIA
Revista:
Frontiers in Plant Science
Editorial:
Frontiers
Referencias:
Lugar: Lausanne; Año: 2015 vol. 5 p. 1 - 13
Resumen:
In the past decade, plant nuclear factor Y (NF-Y) genes have gained
major interest due to their roles in many biological processes in plant
development or adaptation to environmental conditions, particularly in
the root nodule symbiosis established between legume plants and nitrogen
fixing bacteria. NF-Ys are heterotrimeric transcriptional complexes
composed of three subunits, NF-YA, NF-YB, and NF-YC, which bind with
high affinity and specificity to the CCAAT box, a cis element present in
many eukaryotic promoters. In plants, NF-Y subunits consist of gene
families with about 10 members each. In this study, we have identified
and characterized the NF-Y gene families of common bean (Phaseolus
vulgaris), a grain legume of worldwide economical importance and the
main source of dietary protein of developing countries. Expression
analysis showed that some members of each family are up-regulated at
early or late stages of the nitrogen fixing symbiotic interaction with
its partner Rhizobium etli. We also showed that some genes are
differentially accumulated in response to inoculation with high or less
efficient R. etli strains, constituting excellent candidates to
participate in the strain-specific response during symbiosis. Genes of
the NF-YA family exhibit a highly structured intron-exon organization.
Moreover, this family is characterized by the presence of upstream ORFs
when introns in the 5' UTR are retained and miRNA target sites in their
3' UTR, suggesting that these genes might be subjected to a complex
post-transcriptional regulation. Multiple protein alignments indicated
the presence of highly conserved domains in each of the NF-Y families,
presumably involved in subunit interactions and DNA binding. The
analysis presented here constitutes a starting point to understand the
regulation and biological function of individual members of the NF-Y
families in different developmental processes in this grain legume.