INVESTIGADORES
BLANCO Flavio Antonio
artículos
Título:
Insights into post-transcriptional regulation during legume-rhizobia symbiosis
Autor/es:
REYNOSO, MAURICIO; BLANCO, FLAVIO; ZANETTI, MARÍA EUGENIA
Revista:
Plant signaling and behaviour
Editorial:
Landes Biosciences
Referencias:
Lugar: Austin, TX; Año: 2013 vol. 8 p. 1 - 7
ISSN:
1559-2316
Resumen:
During the past ten years, changes in the transcriptome have
been assessed at different stages of the legume-rhizobia
association by the use of DNA microarrays and, more recently,
by RNA sequencing technologies. These studies allowed the
identification of hundred or thousand of genes whose steadystate
mRNA levels increase or decrease upon bacterial infection
or in nodules as compared with uninfected roots.1-7 However,
transcriptome based-approaches do not distinguish between
mRNAs that are being actively translated, stored as messenger
ribonucleoproteins (mRNPs) or targeted for degradation.
Despite that the increase in steady-state levels of an mRNA
does not necessarily correlate with an increase in abundance
or activity of the encoded protein, this information has been
commonly used to select genes that are candidates to play a
role during nodule organogenesis or bacterial infection. Such
criterion does not take into account the post-transcriptional
mechanisms that contribute to the regulation of gene
expression. One of such mechanisms, which has significant
impact on gene expression, is the selective recruitment
of mRNAs to the translational machinery. Here, we review
the post-transcriptional mechanisms that contribute to the
regulation of gene expression in the context of the ecological
and agronomical important symbiotic interaction established
between roots of legumes and the nitrogen fixing bacteria
collectively known as rhizobia.8 In addition, we discuss how the
development of new technologies that allow the assessment of
these regulatory layers would help to understand the genetic
network governing legume rhizobia symbiosis.
1-7 However,
transcriptome based-approaches do not distinguish between
mRNAs that are being actively translated, stored as messenger
ribonucleoproteins (mRNPs) or targeted for degradation.
Despite that the increase in steady-state levels of an mRNA
does not necessarily correlate with an increase in abundance
or activity of the encoded protein, this information has been
commonly used to select genes that are candidates to play a
role during nodule organogenesis or bacterial infection. Such
criterion does not take into account the post-transcriptional
mechanisms that contribute to the regulation of gene
expression. One of such mechanisms, which has significant
impact on gene expression, is the selective recruitment
of mRNAs to the translational machinery. Here, we review
the post-transcriptional mechanisms that contribute to the
regulation of gene expression in the context of the ecological
and agronomical important symbiotic interaction established
between roots of legumes and the nitrogen fixing bacteria
collectively known as rhizobia.8 In addition, we discuss how the
development of new technologies that allow the assessment of
these regulatory layers would help to understand the genetic
network governing legume rhizobia symbiosis.
8 In addition, we discuss how the
development of new technologies that allow the assessment of
these regulatory layers would help to understand the genetic
network governing legume rhizobia symbiosis.