FATTY ACID B-OXIDATION, ITS RELATIONSHIP WITH BOVINE OOCYTE ATP CONTENT, MATURATION AND DEVELOPMENTAL COMPETENCE

BREININGER ELIZABETH; CETICA PABLO; GIL VICTOR; GUTNISKY CYNTHIA

Buenos Aires

Jornada; XVIII Jornadas Anuales Multidisciplinarias; 2016

Sociedad Argentina de Biología

The aim of this work was to evaluate β-oxidation of fatty acids in bovine oocyte in vitro maturation (IVM) and it effect on oocyte ATP content, maturation process and developmental competence. Cumulus-oocyte complexes (COCs) were obtained by aspiration from slaughtered cows ovaries. IVM was performed in 199 + 5% FBS + FSH + LH (Control) supplemented with a β-oxidation inhibitor or stimulator, etomoxir (E) or L-carnitine (L-C), respectively. In vitro fertilization (IVF) and embryo development was performed in mSOF. ATP content was measured in matured-denuded oocytes using a kit based in the luciferin-luciferase reaction. Meiotic maturation was evaluated by the presence of the chromosome plate in metaphase II by Hoechst stain. IVF was evaluated by the cleavage rate and embryo development by the blastocyst rate at day 7 following insemination. COCs matured with E showed a significant decrease in oocyte ATP content and in nuclear maturation rates (p