Redox state in potential bovine cygotes and its relation with capacitation inducers and sperm oxidative metabolism

FERNÁNDEZ S; CÓRDOBA M; MORADO S; CETICA P

CABA

Congreso; 3º Congreso de la Sociedad Argentina de Tecnologías Embrionarias; 2016

Sociedad Argentina de Tecnologías Embrionarias

Mammalian sperm requires to go through a series of events in the female reproductive tract, known as sperm capacitation, to be capable of fertilizing the oocyte. Although heparin is the most commonly used capacitation inducer in vitro, hyaluronic acid has been recently used for capacitation induction as well as for other reproductive biotechnologies such as sperm selection and in vitro fertilization (IVF). The aim of this work was to study the induction of sperm capacitation with heparin or hyaluronic acid and its relation with cleavage rate and redox state in potential bovine embryos. Capacitation was evaluated by chlorotetracicline epifluorescence technique, sperm viability by trypan blue stain and oxygen consumption determined by polarography. For IVF frozen sperm was thawed in modified synthetic oviduct fluid (mSOF), then centrifuged and resuspended to a final concentration of 2x10 6 motile sperm cells/ml. Cumulus-oocyte complexes and sperm samples were co-incubated in mSOF with the addition of bovine serum albumin and heparin or hyaluronic acid at 39°C and 5% CO2 in humidified air during 21 hours. Redox state was determined by RedoxSensor Red CC-1. Data were analyzed by ANOVA and Tukey tests (P