EFECTO DE LA AGREGACIÓN EMBRIONARIA Y COMPLEMENTACIÓN CELULAR SOBRE EL DESARROLLO IN-VITRO DE EMBRIONES PARTENOGÉNICOS BOVINOS

SAVY V; BEVACQUA R; VANS LANDSHOOT G; SALAMONE D

Congreso; Primer Congreso latinoamericano de Reproducción Animal; 2015

Aggregation of zona free cloned embryos (ZF) significantly improves embryo quality during in-vitro development (Gambini et al., 2012). However, it has not yet been reported the effect of somatic cell aggregation on in-vitro embryos development. The objective of this work was to assess whether a discrete number of somatic cells can be integrated to ?aggregated embryos?, and how they affect in-vitro embryo development. To this aim, ZF aggregated embryos (ZFE) development was compared to ZF aggregated and somatic cells-complemented embryos, using either mesenchymal adipose stem cells (ASC) (ZFC-A group) or bovine fibroblasts (BF) (ZFC-F group). A ZP (with zona pellucidae) group injected with ASC was also included. To be able to visualize the cells, ASC were stained with the lipophilic cationic dye CM-DIL while BF were isolated from a transgenic animal carrying pT2/SB-Venus construction. Significant differences were observed between ZFE and ZFC-F groups and also between IA and ZFE (p