HIALURONIC ACID AND PROGESTERONE EFFECT IN BOVINE CRYOPRESERVED SPERM

PÉREZ MARCHETTA G. ,; FILOSA A.; CÓRDOBA M

Jornada; IV Jornadas de INITRA; 2014

Ins. de Investigacion en Reproduccion animal INITRA

 hialuronic acid And progesterone Effect in bovine cryopreserved sperm   Pérez Marchetta G., FILOSA, A., CÓRDOBA, m. Cátedra de Química Biológica, Facultad Ciencias Veterinarias-UBA. Instituto de Investigación y Tecnología en Reproducción Animal Unidad ejecutora UBA-Conicet de Investigaciones en Producción Animal   Heparin (HP) and hyaluronic acid (HA) are glycosaminoglycans present in the female bovine genital tract. HP participates in sperm capacitation, important event for acrosomal reaction, which is physiologically induced by zona pellucida and progesterone. These events are necessary for oocyte fertilization. HP increases intracellular calcium in bovine cells. In mammals, HA is produced by the female genital tract and cumulus oophurus cells. Progesterone also increases intracellular calcium and provides fertilizing potential to human and bovine sperm. The aim of this work was to study HA and progesterone effect as physiological inductors of bovine cryopreserved sperm and their influence on in vitro fertilization. Motility and vigor were observed at 37°C. Sperm concentration was determined by hematocymetry. Viability and acrosome integrity were analyzed by trypan blue stain and interferential differential (DIC) optic microscopy. Capacitation and acrosome reaction was evaluated by chlortetracycline (CTC) epifluorescent stain. Results were analyzed by ANOVA and Tukey test (p<0.05). CTC spermat capacitation increased with HA treatment (32.33 ± 4.12%) respect to control (14.33 ± 5.839%) (p<0.05). The addition of progesterone to capacitated spermatozoa with HA caused an increase in spermatozoa with true acrosome reaction (18.64 ± 7.00%) respect to control (p<0.05). Viability did not change due to treatments (p˃0.05). The present study introduces HA as a glycosaminoglycan that may cause in vitro intracellular changes and capacitation of cryopreserved bovine sperm.