INPA   24560
UNIDAD EJECUTORA DE INVESTIGACIONES EN PRODUCCION ANIMAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Biochemical and morphologic alterations in cooper deficient bovines
Autor/es:
OLIVARES RWI; SHAPIRA A; POSTMA GC; IGLESIAS IE; VALDEZ LB; BREININGER E; MINATEL L
Reunión:
Congreso; VIII Meeting of the Society for Free Radical Biology and Medicine South American Group; 2013
Resumen:
Copper deficiency is an important cattle disease, with several clinical signs due to alterations in copper-dependent enzymes. This deficit is produced by an excess of molybdenum and sulphur in the diet. These elements cause a reduction of copper absorbability. The aim of this work was to determine the effect of copper deficiency on bovine heart. Two groups of five Holstein calves were used: the control group received 9 mg of copper/kgDM in the diet, while the deficient group received 11 mg of molybdenum/kgDM and 3 g of sulphur/kgDM. After 8 months of treatment, copper levels (plasmatic, < 57 µg/dl; hepatic, < 25 ppm), indicative of deficiency, were observed. Animals were sacrificed after 12 months of treatment and myocardial samples were taken. Mitochondrial structure was evaluated using electron microscopy. Copper concentration, lipids oxidation (TBARS), cytochrome c oxidase (COX) and Cu,Zn-superoxide dismutase (Cu,Zn-SOD) activities were measured in myocardial samples. Cardiac copper levels (23%), COX (27%) and Cu,Zn-SOD (38%) activities were reduced in deficient animals. Mitochondrial damage, determined as swelling, cristae and membranes disruption; and lipids oxidation (TBARS, 58%) were increased in deficient animals. Heart COX and Cu,Zn-SOD activities reduction from copper deficient bovines could lead to mitochondrial respiration impairment and damage, with an increase on oxidative damage parameters (TBARS).