Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina

JOSEE LABRIE; ALICIA FERNANDEZ CIRELLI; YANNICK D. N. TREMBLAY; MARIO JACQUES; MARIELA E. SREDNIK; MARIE ARCHAMBAULT; ELIDA R. GENTILINI

FEMS MICROBIOLOGY LETTERS

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2017 vol. 364 p. 1 - 8

0378-1097

Mastitis affects the health and welfare of dairy cows worldwide. Coagulase-negative staphylococci (CNS) are known to formbiofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. A total of 90 CNSisolated from cows with clinical and subclinical mastitis in Argentina from 2008 to 2014 were identified by PCR-RFLP usingthe gap gene. Standard microtiter plate assays were used to assess CNS biofilm formation, and Staphylococcus haemolyticusspecies formed the strongest biofilms. The presence of biofilm-associated genes icaA, bap and aap was detected in a fewisolates, while embP, fbe, atlE and eno were present in the majority of isolates. Genes encoding resistance to β-lactams weredetected among the isolates; blaZ, mecA and mecC were detected in 21, 4 and 1 isolate, respectively. Resistance to macrolidesand lincosamides (n = 6) was attributable to ermB, ermC, mphC or mrsA or a combination of those genes. In this study, weidentified CNS species involved in mastitis and provide information about pathogenicity and antimicrobial resistance,which is essential to design efficient strategies to control mastitis caused by CNS.