Implications of glycolytic and pentose phosphate pathways on the oxidative status and active mitochondria of the porcine oocyte during in vitro maturation

S. CASIRÓ; M. SUTTON-MCDOWALL; G. ALVAREZ; G. ALVAREZ; G. DALVIT; G. DALVIT; P. CETICA; P. CETICA; C. GUTNISKY; C. GUTNISKY; J. THOMPSON; J. THOMPSON; S. CASIRÓ; M. SUTTON-MCDOWALL

THERIOGENOLOGY

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2016 vol. 86 p. 2096 - 2106

0093-691X

The glycolysis and pentose phosphate pathway (PPP) were modulated in porcine cumulus-oocyte complexes (COCs) during in vitro maturation (IVM) by the addition of inhibitors and stimulators of key enzymes of the pathways, with the aim of analizing their participation on the oxidative status, mitochondrial activity and maturation of the oocyte. Glycolysis and PPP in COCs were evaluated by lactate production-glucose uptake and by the Brilliant Cresyl Blue test, respectively. Oocyte oxidative and mitochondrial activities were evaluated by Redox Sensor Red CC-1 and Mito Tracker Green FM, respectively. Oocyte nuclear maturation rate and oxidative and mitochondrial activities decreased in the presence of the pharmacological (NaF) or the physiological (ATP) inhibitors of glycolysis (P