Participation of membrane adenylyl cyclase (mAC) in heparin-induced capacitation in cryopreserved bovine spermatozoa

RICART C; BREININGER E; RODRIGUEZ PC; BECONI M

ANDROLOGIA

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2015 vol. 47 p. 30 - 36

0303-4569

The aim of this work was to study the participation of membrane adenylylcyclase in heparin-induced capacitation in cryopreserved bovine spermatozoa.Sperm suspensions were incubated in Tyrode?s albumin lactate pyruvate mediumin the presence of heparin (10 IU ml1) or forskolin (1?75 lM), a wellknownmembrane adenylyl cyclase activator. The participation of membraneadenylyl cyclase was confirmed using a specific inhibitor, 2′,5′-dideoxyadenosine(6?25 lM). Spermatozoa capacitated with forskolin (25 lM) were incubatedwith bovine follicular fluid to evaluate their ability to undergo acrosome reaction.Capacitation percentages were determined by the fluorescence techniquewith chlortetracycline, and true acrosome reaction was determined by trypanblue and differential interferential contrast. The forskolin concentrationsemployed had no effect on progressive motility or sperm viability. Capacitationvalues induced by 25-lM forskolin treatment (27.80 2.59%) were significantlyhigher respect to the control (4.80 1.30%). The inhibitor 2′,5′-dideoxyadenosineprevented forskolin-induced capacitation and significantly diminishedcapacitation induced by heparin. Follicular fluid induced physiological acrosomereaction in spermatozoa previously capacitated with 25-lM forskolin(P < 0.05). Forskolin acts as a capacitation inducer and involves the participationof membrane adenylyl cyclase as part of the intracellular mechanisms thatlead to capacitation in cryopreserved bovine spermatozoa.