Comparative Analysis of Different Techniques to Determine Motility Parameters in Video Sequences of Ram and Buck Sperm

BUCHELLY IMBACHÍ, FRANCISCO; ISA JARA, RAMIRO; ZALAZAR, LUCÍA; CESARI, ANDREINA; PASTORE, JUAN IGNACIO; BALLARIN, VIRGINIA

Cancun

Conferencia; VIII Latin American Conference on Biomedical Engineering and XLII National Conference on Biomedical Engineering; 2019

International Federation for Medical and Biological Engineering

In this paper we analyzed different techniques, included in the SpermMotility Tracker (SMT) software, used to determine the standard motilityparameters in video sequences of sperm. The commercial Sperm Class Analyzer(SCA) software was used as reference to evaluate the two proposed methods.Two tests were performed: first, samples of ram and buck sperm with a constantframe rate were analyzed and the linear correlation coefficient was foundbetween the variables measured with each method and with the commercialSCA. In the second test, samples of ram sperm were acquired with three differentframe rates and the parameters that depend on the smoothing procedurewere compared. As a result of both tests, the measurements obtained with theSMT were very similar to the ones obtained with the SCA for the most ofvariables. As a particular case, one of the proposed methods showed a slightlybetter performance to measure the amplitude of lateral head oscillation (ALH),but the measures of the beat-cross frequency (BCF) present significant differencesregarding the commercial SCA values.