023 Removal of the decapacitanting protein SPINK3 by interaction with female environment in Mus musculus

NICOLLI, ANABELLA; GRECO, MICAELA BELÉN; GUAGNINI, MARCELO; PEREZ MARTINEZ, SILVINA; CESARI, ANDREINA

Ciudad Autonoma de Buenos Aires

Congreso; Xth Meeting of the Latin American Society for Developmental Biology; 2019

Latin American Society for Developmental Biology

In mammals, sperm capacitation is regulated by decapacitating factors secreted bymale accessory glands. A fter mating, sperm interact with the uterus and oviduct, afundamental step for selection, survival and capacitation. SPINK3 is adecapaitating protein secreted by the seminal vesicle in mouse. This workevaluated the site and kinetics of SPINK3 release fr om the sperm surface. Twoapproaches were conducted: aEpididymal sperm pre incubated with recombinantSPINK3 where incubated with uterine fluid from estrous or metaestrus females andlocalization of SPINK3 was evaluated by immunocytochemistry. b Estrous fe maleswere euthanized 0, 1.5 and 3hs post coitus and the uterus oviduct region wasdissected. SPINK3 was evaluated by immunocytochemistry andimmunohistochemistry. After 15 30 min of incubation with estrous uterine fluid, theproportion of cells with SPINK 3 on the apical region and flagellum was significantlyreduced. For the in vivo test, the sperm obtained from the uterus at time 0 showedSPINK3 signal on head and flagellum, while 1.5h post coitus only the flagellumsignal was observed at the uterus and o viduct. After 3hs sperm were observedinteracting with the oviductal epithelium with no SPINK3 labeling. Our resultssuggest that SPINK3 detaching is a two step process in which head and terminalpiece release are mediated by different female stimulus. The time and the femaleduct section where SPINK3 was lost are in agreement with evidence of theCatSper regulation.