Preliminary Semen Sample Management From Paralichthys Orbignyanus For Sperm Motility Objective Analysis By Using A Novel Open-Source Suite Of Algorithms

ZALAZAR, LUCÍA; BUCHELLY IMBACHÍ, FRANCISCO; GRECO, MICAELA BELÉN; LOPEZ, ANDREA; RADONIC, MARIELA; PASTORE, JUAN IGNACIO; ARISTIZABAL, EDDIE; CESARI, ANDREINA; BALLARIN, VIRGINIA

CABA

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación. Reunión Conjunta de Sociedades de Biociencias.; 2017

SAIC

A novel suit of open-source algorithms for sperm motility evaluationwas previously reported by our group. The proposed methodwas successfully compared with the Microptic􀀳s Sperm Class Analyzer®. Regarding the precision of the system, number of detectedmotile trajectories and the percentage of fragmented paths to measurethe tracking reliability of ram sperm, our method presented abetter performance according to those indicators compared to thecommercial system.Knowledge related to marine 􀀴sh sperm motility is poor. In viewto improve gamete cryopreservation in our country, we performedpreliminary assays to standardize the sample collection by strippingand evaluation of semen quality from the autochthonous 􀀐ounderParalichthys orbignyanus. This species is currently cultured undercaptivity in the Mariculture Experimental Station of National Institutefor Fisheries Research and Development. We performed preliminarymotility measurements by videomicroscopy using the recentlydeveloped algorithm. Sperm movement was recorded at 30 fr/secusing a videocamera attached to a microscope with a phase-contrastobjective. Since 􀀴sh sperm functionality depend on several factors,􀀴rstly, it was necessary to assay sample dilutions in salt waterin order to activate the sperm and to 􀀴nd an appropriate sperm concentrationthat allow detecting individual trajectories. Concentrationshould not be over 30*106 cells/ml to guarantee the correct tracking.Motility parameters of progressive sperm were within 52.4 to 886.7􀀼m/s for VAP, 1.7 to 878.1 􀀼m/s for VSL; 201.3 to 885.5 􀀼m/s forVCL; 0 to 4.9 􀀼m for ALH and 0 to 26.6 Hz for BCF, consistent withmeasurements reported in other marine 􀀴shes such as Sparus aurata.Even though there is not much published in 􀀴sh sperm motilityparameters, these result are promising to evaluate the quality of 􀀴shsemen of interest for aquiculture and eventually to progress withfertility studies as well as cryopreservation protocols