INVESTIGADORES
CESARI Andreina
congresos y reuniones científicas
Título:
Evaluation of motility quality in ram sperm by using a novel open-source suite of algorithms
Autor/es:
BUCHELLY IMBACHÍ, FRANCISCO; ZALAZAR, LUCÍA; PASTORE, JUAN IGNACIO; CESARI, ANDREINA; BALLARIN, VIRGINIA
Lugar:
Cordoba
Reunión:
Congreso; XXI Congreso Argentino de Bioingeniería; 2017
Institución organizadora:
Sociedad Argentina de Bioingenieria
Resumen:
Abstract?In this paper a novel suit of open-source algorithms for sperm motility evaluation is presented. The proposed method first applies a segmentation, separating spermatozoa heads from the background in a binary image. Then, the mass center of each binary object is determined to develop a time tracking. The tracking stage is performed using individual Kalman filters to predict each particle position and a simplified occlusion handling method due to the low size of the particles. The system was compared with the Microptic?s Sperm Class Analyzer® according to the tracking performance, evaluating the precision of the system and the percentage of fragmented paths to measure the tracking reliability. Results show that the proposed method presented a better performance according to those indicators. Finally, motility percentages were found to corroborate the higher number of detected motile trajectories of our method.