INVESTIGADORES
CESARI Andreina
congresos y reuniones científicas
Título:
Rat sperm rosettes: Identification of epididymal proteins involved in sperm rosettes assembling
Autor/es:
MONCLUS MA; CABRILLANA ME; CESARI, A; VINCENTI AE; FORNÉS MW
Lugar:
Villa Carlos Paz, Cordoba, Argentina
Reunión:
Congreso; XLIV Reunion Annual SAIB; 2008
Institución organizadora:
Sociedad Argentina de Investigacion en Bioquimica y Biologia Molecular
Resumen:
In many mammals? species, sperm associate during their epididymal
trip. This association could be related to sperm maturation and
protection during epididymal storage. In the rat these association
were called Rosettes and appeared in the distal cauda. The aim of this
work was isolate proteins from caudal epididymal fluid (EF) with
activity to re associate sperm in vitro trying to mimic the in vivo
phenomenon (in vitro sperm re association test: i.v. Rosette test).
Mature and motile sperm (1.106 cells/ml) was incubated with EF
fractions (1 mg/ml) and the number of Rosettes obtained in vitro was
recorded (expressed as Rosettes number/60 microscopic fields, 400
X). Using gel filtration ?proteins separated by size range (Sephacryl
S200) - and affinity chromatographies ?Glucose/mannose affinity
proteins fractionation (Concanavalin A sepharose) - EF was
fractionated in eight fractions.
All fractions were tested by i.v. Rosettes test and the most active
fraction (with MW from 40 to 80 KDa) and Concanavalin A affinity
were analysed by MALDI-TOF-MS. Two proteins with serine
protease inhibitor activity (serpins) were identified: á1-Antitrypsin
and a new protein with a á1-Antitrypsin like domain. These results
and previous indicate that Rosettes are assembled during the
epididymal trip by several factors: sperm motility inhibition, linking
proteins, proteases and their inhibitors.