INVESTIGADORES
CESARI Andreina
artículos
Título:
Cryopreservation and egg yolk extender components modify the interaction between seminal plasma proteins and the sperm surface
Autor/es:
RAMÍREZ-VASQUEZ, RAFAEL R.A.; CANO, ADRIANA; HOZBOR, FEDERICO A.; CESARI, ANDREINA
Revista:
THERIOGENOLOGY
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Año: 2019
ISSN:
0093-691X
Resumen:
It is known that the addition of SP or SP proteins either before freezing or post thawing show contradictory results on sperm quality and fertility due to the interference between SP and the extender. Thus, the aim of this study was to determine whether egg yolk (EY) interferes with seminal plasma (SP) ability to protect the functionality and fertility of ram sperm during freeze-thawing by modifying the interaction between seminal plasma proteins and the sperm plasma membrane.Ejaculated or epididymal ram sperm collected during the breeding season were incubated with SP in the presence or absence of EY or soybean lecithin-based extenders before cryopreservation. No significant differences were observed after thawing in sperm quality (total and progressive sperm motility, membrane integrity, plasma membrane functionality, percentage of non-capacitated sperm) between the extenders, either in presence or absence of seminal plasma (P≥0.05). The amount of proteins retained by the sperm surface normalized to number of cells was diminished after freeze-thawing compared to their fresh counterparts for all the treatments (P