Combined EGF and Hyaluronic acid supplementation of in vitro maturation medium and its impact on bovine oocyte proteome and competence

RIOS, G; BUSCHIAZZO, J; MUCCI, N; KAISER, G.; CESARI, A; ALBERIO, RH

THERIOGENOLOGY

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2015 vol. 83 p. 874 - 880

0093-691X

The conditions for in vitro oocyte maturation impact on cytoplasmic and nuclear processes in the oocyte. These events are differentially influenced by the nature of the maturation inducer as well as by the presence of intact cumulus in COCs. EGF is the main growth factor promoting oocyte maturation. Also, hyaluronic acid (HA) produced by cumulus cells is known to be responsible for the correct structural and functional organization of the cumulus during oocyte maturation. Therefore, we evaluated the developmental competence of bovine oocytes matured in vitro in a maturation medium supplemented with both EGF and HA, compared to FSH and FBS. In addition, the impact of IVM conditions on the proteomic profile of metaphase II bovine oocytes was analyzed by 2D electrophoresis. Cumulus oocyte complexes were matured in two media: 1) 10 ng/mL EGF, 15 μg/mL HA, 100 μM cysteamine and 2) 0.01 UI/ mL rh-FSH, 10% fetal bovine serum (FBS). The percentages of first polar body and embryo production and the kinetics of embryo development and oocyte proteomic profiles were analyzed. Oocytes matured in the presence of EGF-HA showed an increase (6%, p