EFFECT OF STRESS EXPOSURE IN THE PROGRESSION OF DIABETES. PARTICIPATION OF THE MICROBIOTA

ANA MARÍA GENARO; ROXANA RUBISTEIN; MIRIAM R WALD

Virtual

Congreso; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2020

SAIC

Type 1 diabetes (T1D) is an autoimmune disease characterized by impaired insulinsecretion. Recently, there was an increase in T1D suggesting the participation ofenvironmental factors in its development. It is well known that the microbiota canmodulate the immune system and multiple studies have shown a decrease in themicrobial diversity prior and after the development of the T1D, but the mechanisminvolved remains unknown. In addition, it has been recognized the contribution ofpsychosocial factors in T1D. We have previously shown that chronic stress worsens theprogression of T1D and multiple studies have shown that stress can modify the microbialcomposition. The aim of this project is to determine participation of the microbiota andstress on the development and progression of T1D and the mechanisms involved. Toinduced diabetes, we treated male BALB/c mice with multiple low doses of streptozotocin(stz) and then, the animals were subject to chronic mild stress (CMS) by a dailyapplication of different mild stressors. Fecal samples were collected and genomic DNAwas extracted. 16s Bacteriodetes and 16s Firmicultes (most abundant component of themicrobiota) were measured by qPCR using specific primers. We found an increase in 16sBacteroidetes after CMS exposure in stz treated mice (DIAB + CMS group) compared toall the other treatments (Ctrol, CMS and DIAB groups. One-way ANOVA, p