REGULATION OF PH IN CYSTIC FIBROSIS CELLS BY EGFR

VALDIVIESO A.G.; SANTA COLOMA, T.A .; CLAUZURE, M.; MASSIP-COPIZ, MARÍA M; MORI, C.

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Congreso; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2020

Cystic fibrosis (CF) is a genetic disease caused by mutations inthe CFTR (CF transmembrane conductance regulator) gene. Thepulmonary damage observed in severe patients is the major causeof morbidity and mortality due to chronic infections and unresolvedinflammation. The reduction in the airway surface liquid (ASL) pHis one of the hypotheses that tried to explain the high susceptibilityto lung infections. Together with a reduced bicarbonate transportthrough CFTR, an increase in lactic acid secretion could also explainthe changes in extracellular pH. The aim of the present work was todetermine if the EGFR pathway is involved in the pH regulation inCF cells. Two cellular models were used: IB3-1 cells (bronchial epithelialcells derived from a CF patient with a ΔF508/W1282X CFTRgenotype) and C38 cells (IB3-1 ?corrected? cells). The results reporteda decrease in pH in the extracellular medium culture (p