Epicardial adipose tissue lipidome and lipid metabolism: do circulating markers reflect its behavior in coronary artery disease?

LÓPEZ G; SVILAR L; RUBIO M; SCHREIER L; BERMEJO MF; ANCEL P; MARTIN JC; NAKAJIMA K; BARCHUK M ; MIKSZTOWICZ V; DUTOUR A; GABORIT B; BERG G.

Mar del Plata

Congreso; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2020

Epicardial adipose tissue (EAT), a visceral AT surrounding myocardium and coronary arteries, emerged as an important actor incoronary artery disease (CAD). The increase in its volume is an independent risk factor for CAD. We have previously demonstratedthat lipoprotein lipase (LPL) activity is increased in EAT from CADpatients, potentially contributing to the increase in the tissue volume.EAT composition could contribute to CAD as well. Whether circulating markers reflect EAT behavior is still unknown. Our aim wasto evaluate LPL and glycosylphosphatidylinositol-anchored highdensity lipoprotein-binding protein-1 (GPIHBP1) levels in serum andEAT from CAD patients, as well as tissue and lipoproteins lipidomicprofiles, searching for possible parallelisms.Materials and methods: in serum, EAT and subcutaneous AT (SAT)from patients undergoing coronary artery bypass graft (CAD,n=25)or valve replacement (No CAD,n=25), LPL and GPIHBP1 levelswere evaluated by immunoassays and western blot, respectively.In serum, lipoprotein profile was assessed, and very low and highdensity lipoproteins (VLDL and HDL) were isolated by ultracentrifu-gation. Tissue and isolated lipoproteins lipidomes were evaluatedby UHPLC-MS using a LC C18 column and a Q-Exactive plus massspectrometer, in positive and negative ionization modes.Results: Insulin-resistance markers were higher in CAD (p