Metabolic alterations consequent of chronic photic desynchronization in male mice.

CHIESA JUAN JOSE; PLANO SANTIAGO; ROTA ROSANA; REGUEIRA NICOLE; GOLOMBEK DIEGO

COLONIA

Simposio; XV Latin American Symposium on Chronobiology; 2019

Chronic circadian desynchronization generates metabolic alterations that can lead to abnormal weight home- ostasis. We previously found that exposing C57/BL6j mice under a chronic advancing jet lag (CJL) protocol, which consisted of 6-hour phase advances of the light-dark schedule (LD) every 2 days leads to several altered metabolic markers, such as increased adipose tissue and adipocyte size as well as higher levels of plasma triglycerides. In a further study, we studied circadian glucose homeostasis. Mice under CJL showed increased body weight, together with dampened ad-libitum blood glucose rhythms, with higher values during the ?night? under forced desynchronization. Also, preliminary data showed decreased tolerance to glucose at ?night? in a hyperglycemic clamp test. Triglycerides did not evidence rhythmicity neither under LD, nor under CJL. We hypothesized that mistimed glucose usage (as well as loss of insulin sensitivity, not measured in this work), may lead to altered glucose homeostasis, fat accumulation, and increased body weight gain. This loss of cir- cadian homeostasis in energy balance could emerge from desynchronization between behavioral rhythms, caloric intake, and caloric expenditure.