Evaluation of serum cytokines levels as potential peripheral marker of mild cognitive impairment in women.

MARÍA MICAELA CASTRO; NATALIA ERICA MENITE; MARIO MELCON; MARÍA LAURA PALUMBO; ROMINA PAVON; ANA MARÍA GENARO

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencia SAIC.SAFE.SAB.SAP.AACYTAL.NANOMED-ar 2019; 2019

SAIC, SAFE, SAB, SAP, AACYTAL, NANOMED-ar

Mild Cognitive Impairment (MCI) is a transitional stage between cognitive changes of normal aging and early-stage dementia. MCI is recognized as a pathological condition that typically precedes Alzheiner´s disease. A fraction of 20-40% of MCI individuals will progress to dementia within 3 years following the initial diagnosis. Previously, we demonstrated that the cognitive deficit observed in BALB/c mice exposure to chronic mild stress was correlated with a decrease of IFN-g and an increase of IL-4 in hippocampus and peripheral lymph nodes. The aim of this work was evaluated the levels of cytokines IFN-g, IL-1b, IL-4 and IL-6 in serum of subjects with MCI and control as a possible peripheral marker of cognitive deficit. In this pilot study participated six female subjects (aged 60-70 years) from Junin´s city. The participants were randomly selected taking into account the inclusion and exclusion and criteria according to the protocol approved by the COENOBA and the Central Ethics Committee of the Province of Buenos Aires (exp. 2919-1593/17). The subjects were evaluated by neuropsychological tests following the diagnostic criteria of Petersen. Criteria: the control group showed scores in all cognitive functions evaluated in normal range for patient age and schooling (Z score between -1 and 1). The MCI group (MCI of the amnesic type) showed values equal or less than Z -1.5 in the memory tests. The cytokines levels (pg/ml) were measure in serum by ELISA. We found an increase in IL-4 level (C: 0.7±0.4; MCI: 7.0±1.7; p0.05) in MCI respect to control subjects. We did not find a significant difference in IFN-g (C: 51.4±11.5; MCI: 38.3±2.1), IL-1beta (C: 13.9±1.7; MCI: 19.0±2.3) and IL-6 (C: 5.7±1.6; MCI: 7.3±2.7) between the MCI group compared to control group. We conclude that changes in these cytokines serum levels could be related to the cognitive deficit observed in MCI subjects.