Protective effects of the cellular antioxidant system on the functionality of the immune system in a murine model of hyperthyroidism

KLECHA A.; BARREIRO ARCOS M.L.; MACRI DELBONO R.; ROMEO H.; COSTILLA M.; CREMASCHI G.

Buenos Aires

Congreso; Frontiers in Bioscience 3; 2018

Instituto de Biomedicina de Buenos Aires en conjunto con la Sociedad Max Planck de Alemania (IBioBA-MPSP)

BACKGROUND: Hyperthyroidism is characterized by excessive secretion of triiodothyronine and thyroxine by the thyroid gland. Thyroid hormones (THs) exert actions on growth, metabolism and cellular differentiation. OBJECTIVE: To study the effects of hyperthyroidism on oxidative parameters in murine lymphocytic cells, analyzing their effects on lymphocyte functionality. METHODS: BALB/c mice were treated with T4 (12 mg/l) in drinking water for 30 days or with placebo. Serum hormone levels were quantified by RIA. Reactive oxygen species (ROS) and apoptosis were evaluated by flow cytometry using DCFH-DA and Annexin-V/PI. The expression of antioxidant enzymes and iNOS were analyzed by qPCR and western blot. Enzymatic activity was quantified by spectrophotometry. Nitrite production and lipoperoxidation levels were evaluated by the Griess technique and by the TBARs method. RESULTS: Hyperthyroid animals showed a greater increase in body weight, compatible with higher feed intake, exhibited irritability and anxiety and had dry and brittle hair. Lymphocytes from lymph node (LN) and spleen (S) of hyperthyroid animals showed increased levels of ROS (29.1±2.6% in LN and 26.2±2.1% in S, p