Effects of the cytokines on proliferation on hippocampal adult neural progenitor cells

MORONI ALEJANDRO DAVID; PALUMBO MARÍA LAURA; GENARO ANA MARÍA

Buenos Aires

Congreso; Presentado en la Reunión Conjunta de Sociedades de Biociencias; 2017

SAIC; SAI; SAFE

Thehippocampus, an area involved in learning and memory, is sensitive to theeffects of chronic stress. Cytokines have been shown to affect some behaviour, includingmemory. Moreover, IL-1b, IFN-g and IL-6 had been implicated in psychiatricdisorders. Previously, we found that chronic stress induced a cognitive deficitthat was correlated with a decrease in adult neurogenesis and Th1/Th2imbalance. Recently, we observed a decrease in IFN-g mRNA levels in hippocampus and lymph nodes andincrease IL-4 expression in lymph nodes in stressed mice. On the other hand, adult hippocampalneurogenesis is initiated with the proliferation of neural progenitor cells (NPCs)within the dentate gyrus leading to the formation of new neurons. Regulation ofthis process occurs via numerous secreted factors such as cytokine. The aim of this work was to study the effect of IFN-g, IL-1b, IL-4and IL-6 on the NPCs proliferation. NPCs were obtained from hippocampus ofadult BALB/c mice. NPCs were culture in vitro in optimal conditions with growthfactors. In the last 72 h, each cytokine (0.1, 1 and 50 ng/ml) were added andBrdU 2 h before to fix the culture. The proliferation was measure byimmunocytochemistry using DAPI and anti-BrdU-Cy3. We found that cytokine concentrationof 50 ng/ml inhibit the proliferation of the NPCs respect to control withoutcytokine: IFN-g (95%,p